Virus:Article Title: CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Actin Cell Signaling Cat#4970 Mouse anti-Actin Cell Signaling Cat#3700 Rabbit anti-GFP Chromotek Cat#pabg1 Mouse anti-GFP Santa Cruz Biotech Cat#sc-9996 Mouse anti-GAPDH Santa Cruz Biotech Cat#sc-32233 Rabbit anti-tau oligomer (T22) Rakez Kayed, The University of Texas Medical Branch at Galveston Gift Rabbit anti-tau oligomer (M204) David Eisenberg, University of California, Los Angeles N/A Mouse anti-tau oligomer (TOC1) Nicholas Kanaan, Michigan State University N/A Rabbit anti-tau oligomer (T22-I) EMD Millipore Cat#ABN454-I Rabbit anti-tau oligomer (TTC18) Li Gan, Weill Cornell Medical School Gift Mouse anti-tau (Tau13) Santa Cruz Biotech Cat#21796 Mouse anti-tau (HT7) Thermo Fisher Scientific Cat#MN1000 Rabbit anti-tau DAKO Cat#05-803 Biotin anti-tau, 6-18 antibody (Tau12) Biolegend Cat#806506 Pierce Streptavidin Poly-HRP Thermo Fisher Scientific Cat#21140 Mouse anti-NeuN Biolegend Cat#SIG-39860 Mouse anti-Rbx2 (RNF7) Santa Cruz Biotech Cat#166554 Rabbit anti-ELOB ProteinTech Cat#A0024 Rabbit anti-Histone H3 Cell Signaling Cat#4499 Rabbit anti-PSD95 Cell Signaling Cat#3450 Goat anti-rabbit 680 LICOR Cat#926-32210 Goat anti-mouse 800 LICOR Cat#926-68071 Anti-mouse IgG, HRP-linked Cell Signaling Cat#7076 Anti-rabbit IgG, HRP-linked Cell Signaling Cat#7074 Rabbit anti-FLAG Cell Signaling Cat#14793 Mouse anti-HA Cell Signaling Cat#2367 Mouse anti-PSMA2 Thermo Fisher Scientific Cat#MA5-26149 Mouse anti PSMA1-3,5-7 Abcam Cat#MCP231 Rabbit anti PSMD11 Cell Signaling Cat#14303 Rabbit anti-CUL5 Atlas Antibodies Cat#HPA002185 Bacterial or virus strains DH5alpha Thermo Fisher Scientific Cat#18265017 BL21(DE3) Thermo Fisher Scientific Cat#EC0114 MegaX DH10B T1 R Thermo Fisher Scientific Cat#C640003 Chemicals, peptides, and recombinant proteins StemFlex Media Thermo Fisher Scientific Cat#A3349401 Y-27632 Stem Cell Technologies Cat#72308 Matrigel hESC-Qualified Matrix Corning Cat#354277 Doxycycline hyclate (Dox) Sigma-Aldrich Cat#D9891 Brain-derived neurotrophic factor (BDNF) PeproTech Cat#450-02 Neurotrophin-3 (NT-3) PeproTech Cat#450-03 (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e10, March 5, 2026 e1 Please cite this article in press as: Samelson et al., CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.038 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Zymo Gigaprep kit Zymo Cat#D4204 Poly-D-Lysine Culture Dishes Corning Cat#354550 Universal Mycoplasma Detection Kit ATCC Cat#30-1012K Deposited data Raw and processed data (bulk CRISPR-screen data) This paper N/A Experimental models: Cell lines HEK293T ATCC Cat#CRL-11268 Human WTC11 iPSC with Ngn2 transgene and CRISPRi integration Tian et al. 20 N/A Human WTC11 iPSC with Ngn2 transgene and CRISPRa integration Tian et al. 63 N/A Human WTC11 iPSC with Ngn2 transgene and CRISPRi integration WT/V337M This paper N/A Human WTC11 iPSC with Ngn2 transgene integration V337M/V337M Gilbert et al. 77 N/A Oligonucleotides Primers for sequencing; see Table S1 This paper N/A Primers for qPCR; see Table S2 This paper N/A Recombinant DNA pMD2.G Didier Trono Addgene #12259 pMDLg/pRRE Didier Trono Addgene #12251 pRSV-REV Didier Trono Addgene #12253 pC13N-CLYBL-CAG-dCas9-BFP-KRAB Martin Kampmann Addgene #127968 pMK1334 Martin Kampmann Addgene #127965 pAJS1197 Cul5-3xFlag-T2A-mApple This paper N/A pAJS1203 GFP-3xFlag-T2A-mApple This paper N/A pAJS1205 GFP-Tau-1-50-T2A-mApple This paper N/A pAJS1206 GFP-Tau-40-90-T2A-mApple This paper N/A pAJS1207 GFP-Tau-80-130-T2A-mApple This paper N/A pAJS1208 GFP-Tau-120-170-T2A-mApple This paper N/A pAJS1209 GFP-Tau-160-210-T2A-mApple This paper N/A pAJS1210 GFP-Tau-200-250-T2A-mApple This paper N/A pAJS1211 GFP-Tau-240-290-T2A-mApple This paper N/A pAJS1212 GFP-Tau-280-352-T2A-mApple This paper N/A pAJS1172 GFP-Tau-Metless This paper N/A pAJS1184 GFP-Tau-T2A-mApple This paper N/A pAJS1154 GFP This paper N/A pAJS1149 GFP-Tau This paper N/A pZTC-13-R1 Jizhong Zou Addgene #62196 pZT-C13-L1 Jizhong Zou Addgene #62197 pAJS1224 pET-Duet EloBC Gift of John Gross, University of California, San Francisco Gift pAJS1228 His-Sumo-Tau-1-172 This paper N/A pAJS1229 His-Sumo-Tau-1-176 This paper N/A pIVM_26 Alessio Ciulli Addgene #204501 pAJS1200 SOCS4-3xFlag-T2A-mApple This paper N/A pAJS1216 GFP-Tau-80-130-IRES-mApple This paper N/A (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e10, March 5, 2026 e3 Please cite this article in press as: Samelson et al., CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.038 Article
Recombinant:Article Title: CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Actin Cell Signaling Cat#4970 Mouse anti-Actin Cell Signaling Cat#3700 Rabbit anti-GFP Chromotek Cat#pabg1 Mouse anti-GFP Santa Cruz Biotech Cat#sc-9996 Mouse anti-GAPDH Santa Cruz Biotech Cat#sc-32233 Rabbit anti-tau oligomer (T22) Rakez Kayed, The University of Texas Medical Branch at Galveston Gift Rabbit anti-tau oligomer (M204) David Eisenberg, University of California, Los Angeles N/A Mouse anti-tau oligomer (TOC1) Nicholas Kanaan, Michigan State University N/A Rabbit anti-tau oligomer (T22-I) EMD Millipore Cat#ABN454-I Rabbit anti-tau oligomer (TTC18) Li Gan, Weill Cornell Medical School Gift Mouse anti-tau (Tau13) Santa Cruz Biotech Cat#21796 Mouse anti-tau (HT7) Thermo Fisher Scientific Cat#MN1000 Rabbit anti-tau DAKO Cat#05-803 Biotin anti-tau, 6-18 antibody (Tau12) Biolegend Cat#806506 Pierce Streptavidin Poly-HRP Thermo Fisher Scientific Cat#21140 Mouse anti-NeuN Biolegend Cat#SIG-39860 Mouse anti-Rbx2 (RNF7) Santa Cruz Biotech Cat#166554 Rabbit anti-ELOB ProteinTech Cat#A0024 Rabbit anti-Histone H3 Cell Signaling Cat#4499 Rabbit anti-PSD95 Cell Signaling Cat#3450 Goat anti-rabbit 680 LICOR Cat#926-32210 Goat anti-mouse 800 LICOR Cat#926-68071 Anti-mouse IgG, HRP-linked Cell Signaling Cat#7076 Anti-rabbit IgG, HRP-linked Cell Signaling Cat#7074 Rabbit anti-FLAG Cell Signaling Cat#14793 Mouse anti-HA Cell Signaling Cat#2367 Mouse anti-PSMA2 Thermo Fisher Scientific Cat#MA5-26149 Mouse anti PSMA1-3,5-7 Abcam Cat#MCP231 Rabbit anti PSMD11 Cell Signaling Cat#14303 Rabbit anti-CUL5 Atlas Antibodies Cat#HPA002185 Bacterial or virus strains DH5alpha Thermo Fisher Scientific Cat#18265017 BL21(DE3) Thermo Fisher Scientific Cat#EC0114 MegaX DH10B T1 R Thermo Fisher Scientific Cat#C640003 Chemicals, peptides, and recombinant proteins StemFlex Media Thermo Fisher Scientific Cat#A3349401 Y-27632 Stem Cell Technologies Cat#72308 Matrigel hESC-Qualified Matrix Corning Cat#354277 Doxycycline hyclate (Dox) Sigma-Aldrich Cat#D9891 Brain-derived neurotrophic factor (BDNF) PeproTech Cat#450-02 Neurotrophin-3 (NT-3) PeproTech Cat#450-03 (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e10, March 5, 2026 e1 Please cite this article in press as: Samelson et al., CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.038 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Zymo Gigaprep kit Zymo Cat#D4204 Poly-D-Lysine Culture Dishes Corning Cat#354550 Universal Mycoplasma Detection Kit ATCC Cat#30-1012K Deposited data Raw and processed data (bulk CRISPR-screen data) This paper N/A Experimental models: Cell lines HEK293T ATCC Cat#CRL-11268 Human WTC11 iPSC with Ngn2 transgene and CRISPRi integration Tian et al. 20 N/A Human WTC11 iPSC with Ngn2 transgene and CRISPRa integration Tian et al. 63 N/A Human WTC11 iPSC with Ngn2 transgene and CRISPRi integration WT/V337M This paper N/A Human WTC11 iPSC with Ngn2 transgene integration V337M/V337M Gilbert et al. 77 N/A Oligonucleotides Primers for sequencing; see Table S1 This paper N/A Primers for qPCR; see Table S2 This paper N/A Recombinant DNA pMD2.G Didier Trono Addgene #12259 pMDLg/pRRE Didier Trono Addgene #12251 pRSV-REV Didier Trono Addgene #12253 pC13N-CLYBL-CAG-dCas9-BFP-KRAB Martin Kampmann Addgene #127968 pMK1334 Martin Kampmann Addgene #127965 pAJS1197 Cul5-3xFlag-T2A-mApple This paper N/A pAJS1203 GFP-3xFlag-T2A-mApple This paper N/A pAJS1205 GFP-Tau-1-50-T2A-mApple This paper N/A pAJS1206 GFP-Tau-40-90-T2A-mApple This paper N/A pAJS1207 GFP-Tau-80-130-T2A-mApple This paper N/A pAJS1208 GFP-Tau-120-170-T2A-mApple This paper N/A pAJS1209 GFP-Tau-160-210-T2A-mApple This paper N/A pAJS1210 GFP-Tau-200-250-T2A-mApple This paper N/A pAJS1211 GFP-Tau-240-290-T2A-mApple This paper N/A pAJS1212 GFP-Tau-280-352-T2A-mApple This paper N/A pAJS1172 GFP-Tau-Metless This paper N/A pAJS1184 GFP-Tau-T2A-mApple This paper N/A pAJS1154 GFP This paper N/A pAJS1149 GFP-Tau This paper N/A pZTC-13-R1 Jizhong Zou Addgene #62196 pZT-C13-L1 Jizhong Zou Addgene #62197 pAJS1224 pET-Duet EloBC Gift of John Gross, University of California, San Francisco Gift pAJS1228 His-Sumo-Tau-1-172 This paper N/A pAJS1229 His-Sumo-Tau-1-176 This paper N/A pIVM_26 Alessio Ciulli Addgene #204501 pAJS1200 SOCS4-3xFlag-T2A-mApple This paper N/A pAJS1216 GFP-Tau-80-130-IRES-mApple This paper N/A (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e10, March 5, 2026 e3 Please cite this article in press as: Samelson et al., CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.038 Article
CRISPR:Article Title: CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Actin Cell Signaling Cat#4970 Mouse anti-Actin Cell Signaling Cat#3700 Rabbit anti-GFP Chromotek Cat#pabg1 Mouse anti-GFP Santa Cruz Biotech Cat#sc-9996 Mouse anti-GAPDH Santa Cruz Biotech Cat#sc-32233 Rabbit anti-tau oligomer (T22) Rakez Kayed, The University of Texas Medical Branch at Galveston Gift Rabbit anti-tau oligomer (M204) David Eisenberg, University of California, Los Angeles N/A Mouse anti-tau oligomer (TOC1) Nicholas Kanaan, Michigan State University N/A Rabbit anti-tau oligomer (T22-I) EMD Millipore Cat#ABN454-I Rabbit anti-tau oligomer (TTC18) Li Gan, Weill Cornell Medical School Gift Mouse anti-tau (Tau13) Santa Cruz Biotech Cat#21796 Mouse anti-tau (HT7) Thermo Fisher Scientific Cat#MN1000 Rabbit anti-tau DAKO Cat#05-803 Biotin anti-tau, 6-18 antibody (Tau12) Biolegend Cat#806506 Pierce Streptavidin Poly-HRP Thermo Fisher Scientific Cat#21140 Mouse anti-NeuN Biolegend Cat#SIG-39860 Mouse anti-Rbx2 (RNF7) Santa Cruz Biotech Cat#166554 Rabbit anti-ELOB ProteinTech Cat#A0024 Rabbit anti-Histone H3 Cell Signaling Cat#4499 Rabbit anti-PSD95 Cell Signaling Cat#3450 Goat anti-rabbit 680 LICOR Cat#926-32210 Goat anti-mouse 800 LICOR Cat#926-68071 Anti-mouse IgG, HRP-linked Cell Signaling Cat#7076 Anti-rabbit IgG, HRP-linked Cell Signaling Cat#7074 Rabbit anti-FLAG Cell Signaling Cat#14793 Mouse anti-HA Cell Signaling Cat#2367 Mouse anti-PSMA2 Thermo Fisher Scientific Cat#MA5-26149 Mouse anti PSMA1-3,5-7 Abcam Cat#MCP231 Rabbit anti PSMD11 Cell Signaling Cat#14303 Rabbit anti-CUL5 Atlas Antibodies Cat#HPA002185 Bacterial or virus strains DH5alpha Thermo Fisher Scientific Cat#18265017 BL21(DE3) Thermo Fisher Scientific Cat#EC0114 MegaX DH10B T1 R Thermo Fisher Scientific Cat#C640003 Chemicals, peptides, and recombinant proteins StemFlex Media Thermo Fisher Scientific Cat#A3349401 Y-27632 Stem Cell Technologies Cat#72308 Matrigel hESC-Qualified Matrix Corning Cat#354277 Doxycycline hyclate (Dox) Sigma-Aldrich Cat#D9891 Brain-derived neurotrophic factor (BDNF) PeproTech Cat#450-02 Neurotrophin-3 (NT-3) PeproTech Cat#450-03 (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e10, March 5, 2026 e1 Please cite this article in press as: Samelson et al., CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.038 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Zymo Gigaprep kit Zymo Cat#D4204 Poly-D-Lysine Culture Dishes Corning Cat#354550 Universal Mycoplasma Detection Kit ATCC Cat#30-1012K Deposited data Raw and processed data (bulk CRISPR-screen data) This paper N/A Experimental models: Cell lines HEK293T ATCC Cat#CRL-11268 Human WTC11 iPSC with Ngn2 transgene and CRISPRi integration Tian et al. 20 N/A Human WTC11 iPSC with Ngn2 transgene and CRISPRa integration Tian et al. 63 N/A Human WTC11 iPSC with Ngn2 transgene and CRISPRi integration WT/V337M This paper N/A Human WTC11 iPSC with Ngn2 transgene integration V337M/V337M Gilbert et al. 77 N/A Oligonucleotides Primers for sequencing; see Table S1 This paper N/A Primers for qPCR; see Table S2 This paper N/A Recombinant DNA pMD2.G Didier Trono Addgene #12259 pMDLg/pRRE Didier Trono Addgene #12251 pRSV-REV Didier Trono Addgene #12253 pC13N-CLYBL-CAG-dCas9-BFP-KRAB Martin Kampmann Addgene #127968 pMK1334 Martin Kampmann Addgene #127965 pAJS1197 Cul5-3xFlag-T2A-mApple This paper N/A pAJS1203 GFP-3xFlag-T2A-mApple This paper N/A pAJS1205 GFP-Tau-1-50-T2A-mApple This paper N/A pAJS1206 GFP-Tau-40-90-T2A-mApple This paper N/A pAJS1207 GFP-Tau-80-130-T2A-mApple This paper N/A pAJS1208 GFP-Tau-120-170-T2A-mApple This paper N/A pAJS1209 GFP-Tau-160-210-T2A-mApple This paper N/A pAJS1210 GFP-Tau-200-250-T2A-mApple This paper N/A pAJS1211 GFP-Tau-240-290-T2A-mApple This paper N/A pAJS1212 GFP-Tau-280-352-T2A-mApple This paper N/A pAJS1172 GFP-Tau-Metless This paper N/A pAJS1184 GFP-Tau-T2A-mApple This paper N/A pAJS1154 GFP This paper N/A pAJS1149 GFP-Tau This paper N/A pZTC-13-R1 Jizhong Zou Addgene #62196 pZT-C13-L1 Jizhong Zou Addgene #62197 pAJS1224 pET-Duet EloBC Gift of John Gross, University of California, San Francisco Gift pAJS1228 His-Sumo-Tau-1-172 This paper N/A pAJS1229 His-Sumo-Tau-1-176 This paper N/A pIVM_26 Alessio Ciulli Addgene #204501 pAJS1200 SOCS4-3xFlag-T2A-mApple This paper N/A pAJS1216 GFP-Tau-80-130-IRES-mApple This paper N/A (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e10, March 5, 2026 e3 Please cite this article in press as: Samelson et al., CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.038 Article
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